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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human Complement fragment 3a,C3a ELISA Kit
  • 中文名稱：
    人補(bǔ)體片斷3a(C3a)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08509h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人補(bǔ)體片斷3a(C3a)酶聯(lián)免疫試劑盒（CSB-E08509h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的C3a含量。C3a是一種補(bǔ)體系統(tǒng)蛋白，由C3裂解產(chǎn)生，參與免疫和炎癥反應(yīng)。其研究機(jī)制涉及激活破骨細(xì)胞、調(diào)節(jié)免疫細(xì)胞功能等。C3a在多發(fā)性骨髓瘤骨病等疾病中發(fā)揮關(guān)鍵作用，成為潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 ng/mL-2000 ng/mL，本產(chǎn)品適用于科研領(lǐng)域中對(duì)補(bǔ)體系統(tǒng)激活機(jī)制的研究，如炎癥性疾病動(dòng)物模型評(píng)估、免疫調(diào)控通路探索或藥物干預(yù)效果分析等場(chǎng)景，為體外實(shí)驗(yàn)提供靈敏、穩(wěn)定的檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    C3a
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 ng/mL-2000 ng/mL
  • 靈敏度：
    7.81 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-99
    1:2Average %95
    Range %90-105
    1:4Average %92
    Range %82-105
    1:5Average %95
    Range %90-101
  • 回收率：
    The recovery of human C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9795-100
    EDTA plasma (n=4)9895-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    20002.769 2.777 2.773 2.597
    10002.374 2.381 2.378 2.202
    5001.801 1.790 1.796 1.620
    2501.041 1.094 1.068 0.892
    1250.632 0.652 0.642 0.466
    62.50.461 0.473 0.467 0.291
    31.250.259 0.284 0.272 0.096
    00.175 0.176 0.176 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human C3a antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled C3a antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血漿（抗凝劑） EDTA

樣品信息：人

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 我用CSB-E08509h檢測(cè)正常人和患者的血漿樣本，標(biāo)準(zhǔn)曲線擬合良好，OD值范圍在0.071-0.401

By 李老師