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  4. Rabbit Complement fragment 3a,C3a ELISA Kit

Rabbit Complement fragment 3a,C3a ELISA Kit

  • 中文名稱：
    兔子補(bǔ)體片斷3a(C3a)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09283Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子補(bǔ)體片斷3a(C3a)酶聯(lián)免疫試劑盒（CSB-E09283Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的C3a含量。C3a 是補(bǔ)體系統(tǒng)激活產(chǎn)生的重要過敏毒素。它參與炎癥反應(yīng)、免疫調(diào)節(jié)等生理病理過程。研究機(jī)制方面，其與 C3a 受體結(jié)合，能介導(dǎo)細(xì)胞活化、趨化等。在疾病中過度激活可能引發(fā)不良免疫反應(yīng)，相關(guān)研究或?yàn)榧膊≈委熖峁┬掳悬c(diǎn)。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，適用于兔源實(shí)驗(yàn)?zāi)Ｐ停ㄈ鐢⊙Y、自身免疫疾病或組織損傷模型）中補(bǔ)體系統(tǒng)激活程度的評(píng)估，可幫助研究者分析炎癥反應(yīng)機(jī)制、藥物干預(yù)效果或病理狀態(tài)下C3a的動(dòng)態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    C3a
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %80-100
    1:2Average %96
    Range %85-104
    1:4Average %97
    Range %92-104
    1:8Average %90
    Range %82-98
  • 回收率：
    The recovery of rabbit C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9584-107
    EDTA plasma (n=4)9690-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    4002.591 2.620 2.606 2.460
    2001.889 1.874 1.882 1.736
    1001.328 1.320 1.324 1.178
    500.774 0.752 0.763 0.617
    250.434 0.441 0.438 0.292
    12.50.296 0.290 0.293 0.147
    6.250.254 0.244 0.249 0.103
    00.144 0.148 0.146 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：其他 兔

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： 我用CSB-E09283Rb檢測(cè)正常兔血清中β-TG含量，標(biāo)準(zhǔn)曲線的R2可以做到高達(dá)0.9997，標(biāo)準(zhǔn)曲線符合要求，樣品OD值其中之一0.301、0.306，可以做到平行，檢測(cè)結(jié)果準(zhǔn)確且完美。

By 王老師