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  4. Rat Complement fragment 3a,C3a ELISA Kit

Rat Complement fragment 3a,C3a ELISA Kit

  • 中文名稱：
    大鼠補體片斷3a(C3a)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08510r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠補體片斷3a(C3a)酶聯(lián)免疫試劑盒（CSB-E08510r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3a含量。C3a是補體系統(tǒng)的重要成分，由C3蛋白裂解產(chǎn)生，參與免疫和炎癥反應。其研究機制涉及C3a與細胞表面受體的相互作用，激活下游信號通路，調節(jié)免疫細胞功能。C3a在多種疾病中發(fā)揮作用，如糖尿病腎病和多發(fā)性骨髓瘤骨病，成為潛在的治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，本試劑盒檢測靈敏度覆蓋1.56 ng/mL至100 ng/mL寬動態(tài)范圍，可穩(wěn)定識別樣本中痕量目標蛋白。通過優(yōu)化的預包被抗體與酶標記檢測抗體協(xié)同作用，確保檢測結果的高特異性和重復性，適用于科研場景中炎癥機制研究、免疫相關疾病動物模型評估或藥物干預實驗中C3a動態(tài)變化的監(jiān)測，為探索補體系統(tǒng)在生理病理中的作用提供可靠工具。實驗操作簡便，兼容常規(guī)實驗室設備，配套標準品及檢測緩沖液，支持快速完成樣本檢測與數(shù)據(jù)分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    C3a
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 88  
    Range % 80-97  
    1:10 Average % 95  
    Range % 92-102  
    1:20 Average % 96  
    Range % 90-102  
    1:40 Average % 93  
    Range % 90-96  
  • 回收率：
    The recovery of rat C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-97  
    EDTA plasma (n=4) 101 95-107  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.588 2.598 2.593 2.411  
    50 1.948 1.903 1.926 1.744  
    25 1.431 1.468 1.450 1.268  
    12.5 1.009 1.051 1.030 0.848  
    6.25 0.624 0.637 0.631 0.449  
    3.12 0.414 0.438 0.426 0.244  
    1.56 0.279 0.291 0.285 0.103  
    0 0.181 0.183 0.182 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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