Human Creatine Kinase MM isoenzyme(CK-MM) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人肌酸激酶同工酶MM(CK-MM)酶聯(lián)免疫試劑盒（CSB-E18038h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CK-MM含量。CK-MM是一種主要存在于骨骼肌和心臟中的肌酸激酶同工酶，參與能量代謝和肌肉收縮。研究顯示，CK-MM在肌肉損傷和心肌梗死后水平升高，可用于相關(guān)疾病的診斷。其研究機制涉及在肌肉損傷或心肌梗死后，CK-MM從細胞中釋放到血液中，通過檢測血液中的CK-MM水平來診斷疾病。試劑盒檢測范圍為3.12 mU/mL-200 mU/mL，可應(yīng)用于運動醫(yī)學(xué)研究、肌肉損傷機制探索、藥物干預(yù)效果評估等科研領(lǐng)域，為肌肉代謝相關(guān)疾病的分子機制研究提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CK-MM
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 mU/mL-200 mU/mL
靈敏度：

0.78 mU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	111
	Range %	99-114
1:4	Average %	90
	Range %	83-94
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of human CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	104	91-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.