Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠肌酸激酶同工酶MM(CK-MM)酶聯(lián)免疫試劑盒（CSB-E14405r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌中。它可作為診斷急性心肌梗死等疾病的重要指標(biāo)，反映心肌和骨骼肌損傷情況。研究機(jī)制方面，通過檢測血液中CK-MM活性變化，能輔助判斷病情嚴(yán)重程度與預(yù)后，為臨床治療提供關(guān)鍵依據(jù)。試劑盒檢測范圍為7.8 μU/mL-500 μU/mL，適用于運(yùn)動(dòng)醫(yī)學(xué)、肌肉疾病模型構(gòu)建、藥物毒理評(píng)估等科研場景中CK - MM的動(dòng)態(tài)監(jiān)測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CK-MM
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 μU/mL-500 μU/mL
靈敏度：

1.95 μU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	105
	Range %	101-109
1:200	Average %	87
	Range %	84-90
1:400	Average %	102
	Range %	98-106
1:800	Average %	105
	Range %	101-109

回收率：

The recovery of rat CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	85-90

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.