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  4. Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

  • 中文名稱：
    小鼠肌酸激酶同工酶MM(CK-MM)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14407m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肌酸激酶同工酶MM(CK-MM)酶聯(lián)免疫試劑盒（CSB-E14407m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌和心肌中，在臨床上是診斷急性心肌梗死等疾病的重要指標。研究機制方面，通過檢測血液中CK-MM的含量變化，可反映心肌或骨骼肌細胞損傷情況，在心肌梗死時其水平會快速升高。試劑盒檢測范圍為0.156 mU/mL-10 mU/mL，適用于基礎(chǔ)科研中骨骼肌損傷模型評估、運動生理代謝研究、肌肉相關(guān)疾病機制探索等場景，例如通過監(jiān)測血清CK-MM濃度變化研究肌肉再生能力或病理損傷程度。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    CK-MM
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.156 mU/mL-10 mU/mL
  • 靈敏度：
    0.039 mU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %83-98
    1:2Average %98
    Range %92-103
    1:4Average %104
    Range %98-108
    1:8Average %93
    Range %87-99
  • 回收率：
    The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-99
    EDTA plasma (n=4)10095-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    102.420 2.566 2.493 2.343
    51.708 1.855 1.782 1.632
    2.50.970 1.002 0.986 0.836
    1.250.556 0.601 0.579 0.429
    0.6250.359 0.369 0.364 0.214
    0.3120.270 0.278 0.274 0.124
    0.1560.197 0.194 0.196 0.046
    00.155 0.145 0.150 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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