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  4. Human D-Dimer,D2D ELISA Kit

Human D-Dimer,D2D ELISA Kit

  • 中文名稱：
    人D二聚體(D2D)酶聯免疫試劑盒
  • 貨號：
    CSB-E05175h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人D二聚體(D2D)酶聯免疫試劑盒（CSB-E05175h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的D2D含量。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，靈敏度為3.9 ng/mL。適用于基礎醫(yī)學研究，例如通過檢測動物模型或體外實驗中的D二聚體水平，探究抗凝藥物療效、評估血栓性疾病干預策略，或分析纖溶系統在炎癥、腫瘤等病理狀態(tài)中的作用機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    D2D
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 80-95  
    1:2 Average % 96  
    Range % 91-100  
    1:4 Average % 102  
    Range % 92-110  
    1:8 Average % 92  
    Range % 84-98  
  • 回收率：
    The recovery of human D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 80-96  
    EDTA plasma (n=4) 95 90-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.695 2.498 2.597 2.457  
    500 2.289 2.138 2.214 2.074  
    250 1.544 1.486 1.515 1.375  
    125 0.813 0.783 0.798 0.658  
    62.5 0.415 0.401 0.408 0.268  
    31.2 0.279 0.274 0.277 0.137  
    15.6 0.217 0.211 0.214 0.074  
    0 0.141 0.138 0.140 　  
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human D2D antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled D2D antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

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