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Mouse D-Dimer,D2D ELISA Kit

  • 中文名稱:
    小鼠D二聚體(D2D)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13584m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠D二聚體(D2D)酶聯(lián)免疫試劑盒(CSB-E13584m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的D2D含量。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL,廣泛應(yīng)用于心血管疾病模型、凝血功能障礙機(jī)制研究、藥物作用評(píng)估等基礎(chǔ)科研領(lǐng)域,尤其適合在小鼠模型中動(dòng)態(tài)監(jiān)測(cè)凝血系統(tǒng)活化程度或評(píng)價(jià)抗血栓藥物干預(yù)效果,為實(shí)驗(yàn)室提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫:
    D2D
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.81 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %91
    Range %86-98
    1:200Average %97
    Range %92-105
    1:400Average %93
    Range %87-98
    1:800Average %95
    Range %87-103
  • 回收率:
    The recovery of mouse D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9992-105
    EDTA plasma (n=4)9792-102
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.519 2.419 2.469 2.300
    10001.801 1.701 1.751 1.582
    5001.122 1.082 1.102 0.933
    2500.674 0.664 0.669 0.500
    1250.438 0.428 0.433 0.264
    62.50.355 0.345 0.350 0.181
    31.250.289 0.284 0.287 0.118
    00.170 0.168 0.169  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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