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中文名稱:大鼠D二聚體(D2D)酶聯(lián)免疫試劑盒
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貨號:CSB-E12984r
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠D二聚體(D2D)酶聯(lián)免疫試劑盒(CSB-E12984r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細胞裂解物樣本中的D2D含量。大鼠D二聚體(D2D)是纖維蛋白單體交聯(lián)后經(jīng)纖溶酶水解產(chǎn)生的特異性降解產(chǎn)物,是纖溶過程的標記物。其在血栓形成和溶解過程中扮演重要角色,其檢測對于評估血栓性疾病具有重要意義,可用于臨床診斷和科研研究。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL,本產(chǎn)品在凝血功能研究、血栓相關疾病模型建立及藥物作用機制探索中具有重要科研價值,適用于心血管疾病研究、抗凝藥物開發(fā)、炎癥反應評估等科研場景。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:D2D
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates, cell lysates
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檢測范圍:7.8 ng/mL-500 ng/mL
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靈敏度:1.95 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 95 Range % 89-99 1:4 Average % 90 Range % 86-94 1:8 Average % 102 Range % 98-106 -
回收率:
The recovery of rat D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 85 81-92 EDTA plasma (n=4) 89 85-98 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 500 2.465 2.374 2.420 2.238 250 1.661 1.761 1.711 1.529 125 1.052 1.145 1.099 0.917 62.5 0.683 0.702 0.693 0.511 31.2 0.497 0.459 0.478 0.296 15.6 0.321 0.319 0.320 0.138 7.8 0.258 0.247 0.253 0.071 0 0.184 0.179 0.182 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Gut microbial co-metabolite 2-methylbutyrylcarnitine exacerbates thrombosis via binding to and activating integrin a2b1 K Huang, Z Li, X He, J Dai, B Huang, Y Shi, D Fan, Z Zhang, Y Liu, N Li, Z Zhang, J Peng,Cell Metabolism,2024
- An intelligent DNA nanodevice for precision thrombolysis J Yin,Nature materials,2024
- A Novel Insight Into Mechanism of Derangement of Coagulation Balance: Interactions of Quantum Dots with Coagulation-Related Proteins L Zhang,Research Square,2021
- Therapeutic targeting of extracellular DNA improves the outcome of intestinal ischemic reperfusion injury in neonatal rats Boettcher M.et al,Sci Rep,2017
- Early diagnosis of testicular torsion in rats by measuring plasma d-dimer levels: comparative study with epididymitis. Yilmaz E.et al,J Pediatr Surg.,2015
- pH gradient difference around ischemic brain tissue can serve as a trigger for delivering polyethylene glycol-conjugated urokinase nanogels Cui W. et al,J Control Release,2016
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相關問答
Hello, is this product available at 150 $ and how much will it cost inclusive of all taxes.? How many experiments could be performed with this. Thanks in advance
The standard is S0-S7 and we suggest do duplicates for standard.Then this kit of 24T can do 4 tests.If single wells for standard,then this kit of 24T can do 16 tests.
For one 24T kit,the total value you will pay us is $150.0.The import duties will be charged by your country.We are not sure how much is it.
If any further questions,pls let us know.
I am intered in evaluating this kit, do you usually see levels in unspiked samples?
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