Human Epinephrine/Adrenaline,EPI ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人腎上腺素(EPI)酶聯(lián)免疫試劑盒（CSB-E08677h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的EPI含量。試劑盒檢測(cè)范圍為2 pg/mL-400 pg/mL，適用于神經(jīng)生物學(xué)、內(nèi)分泌學(xué)及藥理學(xué)等領(lǐng)域的基礎(chǔ)研究，例如應(yīng)激狀態(tài)下激素分泌機(jī)制探索、心血管疾病模型中的激素動(dòng)態(tài)監(jiān)測(cè)，或藥物干預(yù)對(duì)腎上腺功能影響的評(píng)價(jià)等科研場(chǎng)景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

EPI
種屬：

Homo sapiens (Human)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

2 pg/mL-400 pg/mL
靈敏度：

1 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-96
1:8	Average %	90
	Range %	84-98

回收率：

The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	80-94
EDTA plasma (n=4)	98	92-107