Mouse Epinephrine/Adrenaline,EPI ELISA Kit
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中文名稱:小鼠腎上腺素(EPI)酶聯(lián)免疫試劑盒
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貨號:CSB-E08679m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠腎上腺素(EPI)酶聯(lián)免疫試劑盒(CSB-E08679m)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的EPI含量。小鼠腎上腺素(EPI)是腎上腺分泌的一種激素,對心血管、代謝和應(yīng)激反應(yīng)等生理過程至關(guān)重要。研究其作用機(jī)制,有助于揭示相關(guān)疾病如心血管疾病和代謝綜合征的發(fā)病機(jī)制,并為疾病治療提供新思路。試劑盒檢測范圍為0.78 ng/ml-50 ng/ml,適用于多種科研場景,包括應(yīng)激生理學(xué)模型中激素動(dòng)態(tài)變化的監(jiān)測、藥物或環(huán)境因素對交感 - 腎上腺軸影響的評估,以及代謝性疾病或心血管研究中EPI調(diào)控機(jī)制的探索。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:EPI
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates.
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檢測范圍:0.78 ng/ml-50 ng/ml.
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靈敏度:0.2 ng/ml.
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Neuroscience
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
84
Range %
80-92
1:2
Average %
87
Range %
85-100
1:4
Average %
85
Range %
82-99
1:8
Average %
89
Range %
86-98
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回收率:
The recovery of mouse EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
85
82-90
EDTA plasma (n=4)
87
83-96
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
50
0.180
0.174
0.177
25
0.307
0.317
0.312
12.5
0.485
0.508
0.496
6.25
0.750
0.729
0.740
3.12
1.228
1.301
1.265
1.56
1.972
1.953
1.963
0.78
2.565
2.484
2.524
0
2.916
2.825
2.871
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Heat exposure promotes sarcopenia via gut microbiota‐derived metabolites YF Guo, ZY Liu, M Zhou, WH Kuang, Y Liu, Y Huang,Aging Cell,/
- Papel de la nedilación de PCK1 en la gluconeogénesis hepática U Fernández Paz,Universidade de Santiago de Compostela,2024
- Exercise accelerates recruitment of CD8+ T cell to promotes anti-tumor immunity in lung cancer via epinephrine SN Miao,BMC cancer,2024
- Neddylation of phosphoenolpyruvate carboxykinase 1 controls glucose metabolism MJ Gonzalez-Rellan,Cell metabolism,2023
- Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota Y Liu,Nature communications,2023
- Methionine adenosyltransferase 1a antisense oligonucleotides activate the liver-brown adipose tissue axis preventing obesity and associated hepatosteatosis D Saenz de Urturi Indart,Nature communications,2022
- Methionine adenosyltransferase 1a antisense oligonucleotides activate the liver-brown adipose tissue axis preventing obesity and associated hepatosteatosis D Sáenz de Urturi,Nature communications,2022
- The α2-adrenergic receptor pathway modulating depression influences the risk of arterial thrombosis associated with BDNFVal66Met polymorphism L Sandrini,Biomedicine & Pharmacotherapy,2022
- The Effects of Furosemide on Behavioral and Hormonal Parameters in Male and Female Mice Subjected to Immobilization and Cold-Water Stress BH Ali,Journal of Experimental Pharmacology,2021
- Aminergic status in the brain of urokinase gene-deficient mice with malignant tumors growing in presence of chronic neurogenic pain YA Pogorelova,Journal of Clinical Oncology,2021
- Melatonin ameliorates SGLT2 inhibitor-induced diabetic ketoacidosis by inhibiting lipolysis and hepatic ketogenesis in type 2 diabetic mice Jae-Hyung Park, et al,Journal of Pineal Research,2019
- Acute tobacco smoke exposure exacerbates the inflammatory response to corneal wounds in mice via the sympathetic nervous system Xiao C, et al,Acta Crystallographica Section F-Structural Biology Communications,2019
- AMP-Activated Protein Kinase (AMPK) Regulates Energy Metabolism through Modulating Thermogenesis in Adipose Tissue Lingyan Wu.et al,ORIGINAL RESEARCH ARTICLE,2018
- Activation of RAW 264.7 cells by a polysaccharide isolated from Antarctic bacterium Pseudoaltermonas sp. S-5 Jing Li, et al,Carbohydrate Polymers,2015
- Intestinal gluconeogenesis is crucial to maintain a physiological fasting glycemia in the absence of hepatic glucose production in mice Penhoat A et al,Metabolism,2013
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