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Mouse Epinephrine/Adrenaline,EPI ELISA Kit

  • 中文名稱:
    小鼠腎上腺素(EPI)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08679m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠腎上腺素(EPI)酶聯(lián)免疫試劑盒(CSB-E08679m)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的EPI含量。小鼠腎上腺素(EPI)是腎上腺分泌的一種激素,對心血管、代謝和應(yīng)激反應(yīng)等生理過程至關(guān)重要。研究其作用機(jī)制,有助于揭示相關(guān)疾病如心血管疾病和代謝綜合征的發(fā)病機(jī)制,并為疾病治療提供新思路。試劑盒檢測范圍為0.78 ng/ml-50 ng/ml,適用于多種科研場景,包括應(yīng)激生理學(xué)模型中激素動(dòng)態(tài)變化的監(jiān)測、藥物或環(huán)境因素對交感 - 腎上腺軸影響的評估,以及代謝性疾病或心血管研究中EPI調(diào)控機(jī)制的探索。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    EPI
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates.
  • 檢測范圍:
    0.78 ng/ml-50 ng/ml.
  • 靈敏度:
    0.2 ng/ml.
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-92

    1:2

    Average %

    87

    Range %

    85-100

    1:4

    Average %

    85

    Range %

    82-99

    1:8

    Average %

    89

    Range %

    86-98

  • 回收率:

    The recovery of mouse EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    85

    82-90

    EDTA plasma (n=4)

    87

    83-96

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    50

    0.180

    0.174

    0.177

    25

    0.307

    0.317

    0.312

    12.5

    0.485

    0.508

    0.496

    6.25

    0.750

    0.729

    0.740

    3.12

    1.228

    1.301

    1.265

    1.56

    1.972

    1.953

    1.963

    0.78

    2.565

    2.484

    2.524

    0

    2.916

    2.825

    2.871

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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