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Rat Epinephrine/Adrenaline,EPI ELISA Kit

  • 中文名稱(chēng):
    大鼠腎上腺素(EPI)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08678r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠腎上腺素(EPI)酶聯(lián)免疫試劑盒(CSB-E08678r)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的EPI含量。大鼠腎上腺素(EPI)是腎上腺髓質(zhì)分泌的兒茶酚胺激素,參與調(diào)節(jié)心血管、代謝和呼吸系統(tǒng)功能。其作用機(jī)制涉及激活α和β腎上腺素能受體,調(diào)節(jié)心率、血壓、血糖和脂肪分解等生理過(guò)程。試劑盒檢測(cè)范圍為1 pg/mL-200 pg/mL,該產(chǎn)品可滿(mǎn)足神經(jīng)生物學(xué)、藥理學(xué)及內(nèi)分泌學(xué)等領(lǐng)域的基礎(chǔ)研究需求,例如探索應(yīng)激狀態(tài)下激素釋放機(jī)制、心血管疾病模型中交感神經(jīng)活性評(píng)估,或代謝綜合征相關(guān)動(dòng)物實(shí)驗(yàn)的EPI動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    EPI
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    1 pg/mL-200 pg/mL
  • 靈敏度:
    0.5 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 90-103  
    1:2 Average % 90  
    Range % 85-96  
    1:4 Average % 94  
    Range % 89-99  
    1:8 Average % 85  
    Range % 80-90  
  • 回收率:
    The recovery of rat EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 91 85-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.203 0.202 0.203    
    50 0.378 0.351 0.365    
    16 0.581 0.537 0.559    
    4 0.945 1.012 0.979    
    1 1.416 1.493 1.455    
    0 2.241 2.367 2.304    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    EPI是一種激素,主要由腎上腺分泌,具有增強(qiáng)心臟功能、提高血糖水平等生理作用。最新研究表明,EPI與多種疾病有關(guān),如心血管疾病、代謝綜合征、糖尿病等。一些研究還表明,EPI還可能參與腫瘤的發(fā)生和發(fā)展。未來(lái)的研究將繼續(xù)探討EPI在疾病中的作用機(jī)制,以及開(kāi)發(fā)針對(duì)EPI的治療方法。