Human Estradiol,E2 ELISA Kit
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中文名稱:
人雌二醇(E2)酶聯(lián)免疫試劑盒
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貨號(hào):
CSB-E05108h
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規(guī)格:
96T/48T
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價(jià)格:
¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
人雌二醇(E2)酶聯(lián)免疫試劑盒(CSB-E05108h)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿.樣本中的E2含量。E2 作為一個(gè)靶點(diǎn)備受關(guān)注。在它在相關(guān)生理或病理過程中可能發(fā)揮關(guān)鍵作用。研究機(jī)制上,可能參與特定的信號(hào)通路或分子互作,通過對(duì) E2 進(jìn)行深入研究,有望揭示疾病發(fā)病機(jī)制,為開發(fā)針對(duì)性的治療方法和藥物提供理論依據(jù)。試劑盒檢測(cè)范圍為50 pg/ml-1200 pg/ml.,靈敏度為40 pg/ml.。適用于實(shí)驗(yàn)室環(huán)境下評(píng)估內(nèi)分泌調(diào)控機(jī)制、生殖生物學(xué)研究、藥物對(duì)激素水平的影響分析,以及婦科疾病或代謝綜合征相關(guān)基礎(chǔ)科研。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:
N/A
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縮寫:
E2
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種屬:
Homo sapiens (Human)
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樣本類型:
serum, plasma.
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檢測(cè)范圍:
50 pg/ml-1200 pg/ml.
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靈敏度:
40 pg/ml.
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反應(yīng)時(shí)間:
1-5h
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樣本體積:
50-100ul
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檢測(cè)波長(zhǎng):
450 nm
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研究領(lǐng)域:
Signal Transduction
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測(cè)定原理:
quantitative
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測(cè)定方法:
Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<15% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| | | | | | |
pg/ml | OD1 | OD2 | Average | | | |
0 | 1.952 | 1.834 | 1.893 | | | |
50 | 1.493 | 1.567 | 1.530 | | | |
120 | 1.045 | 1.028 | 1.037 | | | |
250 | 0.679 | 0.629 | 0.654 | | | |
500 | 0.419 | 0.421 | 0.420 | | | |
1200 | 0.234 | 0.243 | 0.239 | | | |
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本試劑盒所含材料:
- A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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本試劑盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理:
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貨期:
3-5 working days
引用文獻(xiàn)
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The flavonoid hyperoside attenuates the toxic effect of cisplatin on the human ovarian granulosa cells: in vitro model study
EM Elmorsy, HA Al Doghaither,Toxicology Mechanisms and Methods,2025
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Hyperoside Attenuates the Toxic Effect of Cisplatin on the Human Ovarian Granulosa Cells: Invitro Model Study
EM Elmorsy, HA Al Doghaither, AB Al-Ghafari,preprints.org,2024
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Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
M Wu,Nature aging,2024
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Adipose triglyceride lipase gene expression in peripheral blood mononuclear cells of subjects with obesity and its association with insulin resistance, inflammation and lipid accumulation in liver
S Ezzati,Obesity science & practice,2024
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METTL3 drives NSCLC metastasis by enhancing CYP19A1 translation and oestrogen synthesis
W Meng,Cell and Bioscience,2024
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Bisphenol A and its analogue bisphenol S exposure reduce estradiol synthesis via the ROS-mediated PERK/ATF4 signaling pathway
Z Li,Food and chemical toxicology,2023
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MicroRNA Let-7i inhibits granulosa-luteal cell proliferation and estradiol biosynthesis by directly targeting IMP2
X Xu,Reproductive biomedicine online,2023
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Malvidin-3-O-Glucoside Ameliorates Cadmium-Mediated Cell Dysfunction in the Estradiol Generation of Human Granulosa Cells
S Liang,Nutrients,2023
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Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling
Y Wu,Free radical biology & medicine,2023
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Fetuin B alleviates testosterone propionate-induced oxidative stress and mitochondrial dysfunction in KGN cells by upregulating the TGFR2/SMAD3 pathway
Y Gao,ResearchSquare,2022
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OI Kit,/,2022
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When male seahorses take the female contraceptive pill …
NP D\'Alvise,Environmental Science and Pollution Research,2020
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In vitro and in vivo approaches for identifying the role of aryl hydrocarbon receptor in the development of nonalcoholic fatty liver disease
Xiang-Yu Zhu, et al,Toxicology Letters,2019
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Cadmium exposure alters steroid receptors and proinflammatory cytokine levels in endothelial cells in vitro: a potential mechanism of endocrine disruptor atherogenic effect
Fittipaldi S, et al,Journal of endocrinological investigation,2018
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Effect of Metformin and Flutamide on Insulin, Lipogenic and Androgen-Estrogen Signaling and Cardiometabolic Risk in a PCOS-prone Rodent Model
Maria Kupreeva.et al,American Journal of Physiology-Endocrinology and Metabolism,2018
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Prenatal high estradiol exposure induces sex-specific and dietarily reversible insulin resistance through decreased hypothalamic INSR
Wang HH.et al,Endocrinology,2017
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Estrogen is an important mediator of mast cell activation in ovarian endometriomas
Zhu T.et al,Reproduction.,2017
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Highly Sensitive Detection of Hormone Estradiol E2 Using Surface-Enhanced Raman Scattering Based Immunoassays for the Clinical Diagnosis of Precocious Puberty
Rui Wang.et al,ACS applied materials & interfaces,2016
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The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice
Yuehan Zhao, Yongdong Zhang, Fang Pan,Centr Eur J Immunol ,2015
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Retinoic acid has the potential to suppress endometriosis development
Yamagata Y. et al,J Ovarian Res,2015
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