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Human Estradiol,E2 ELISA Kit

  • 中文名稱:
    人雌二醇(E2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E05108h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人雌二醇(E2)酶聯(lián)免疫試劑盒(CSB-E05108h)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿.樣本中的E2含量。E2 作為一個(gè)靶點(diǎn)備受關(guān)注。在它在相關(guān)生理或病理過程中可能發(fā)揮關(guān)鍵作用。研究機(jī)制上,可能參與特定的信號(hào)通路或分子互作,通過對(duì) E2 進(jìn)行深入研究,有望揭示疾病發(fā)病機(jī)制,為開發(fā)針對(duì)性的治療方法和藥物提供理論依據(jù)。試劑盒檢測(cè)范圍為50 pg/ml-1200 pg/ml.,靈敏度為40 pg/ml.。適用于實(shí)驗(yàn)室環(huán)境下評(píng)估內(nèi)分泌調(diào)控機(jī)制、生殖生物學(xué)研究、藥物對(duì)激素水平的影響分析,以及婦科疾病或代謝綜合征相關(guān)基礎(chǔ)科研。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    E2
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma.
  • 檢測(cè)范圍:
    50 pg/ml-1200 pg/ml.
  • 靈敏度:
    40 pg/ml.
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    0

    1.952

    1.834

    1.893

    50

    1.493

    1.567

    1.530

    120

    1.045

    1.028

    1.037

    250

    0.679

    0.629

    0.654

    500

    0.419

    0.421

    0.420

    1200

    0.234

    0.243

    0.239

  • 本試劑盒所含材料:
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    雌二醇(Estradiol,E2)是一種雌激素類固醇激素,廣泛存在于雌性生物體內(nèi)。它通過與細(xì)胞表面的雌激素受體結(jié)合來調(diào)節(jié)細(xì)胞的生長(zhǎng)、分化和代謝等生理過程,包括生殖發(fā)育、乳腺發(fā)育、內(nèi)分泌平衡、骨骼代謝、心血管健康和認(rèn)知功能等。最新的研究發(fā)現(xiàn),E2還具有對(duì)中樞神經(jīng)系統(tǒng)和免疫系統(tǒng)的影響,如對(duì)抗自身免疫疾病、改善老年認(rèn)知障礙等具有潛在的治療作用。E2的異常水平與許多疾病的發(fā)生和發(fā)展有關(guān),例如乳腺癌、卵巢癌、骨質(zhì)疏松、心血管疾病、糖尿病和自身免疫疾病等。因此,E2的測(cè)量在臨床診斷和治療中具有重要的意義。