Rabbit Estradiol,E2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子雌二醇(E2)酶聯(lián)免疫試劑盒（CSB-E06915Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的E2含量。E2 是重要靶點(diǎn)，在多種生物過程如病毒復(fù)制、細(xì)胞周期調(diào)控等有作用。研究機(jī)制方面，圍繞其與配體結(jié)合模式、信號(hào)傳導(dǎo)通路開展，探索阻斷其活性以抑制病毒感染、腫瘤生長等，有望為相關(guān)疾病治療提供新思路和策略。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，適用于科研場景中兔類生殖內(nèi)分泌機(jī)制研究、環(huán)境激素干擾評(píng)估、藥理學(xué)實(shí)驗(yàn)的激素動(dòng)態(tài)監(jiān)測，以及畜牧領(lǐng)域繁殖性能相關(guān)基礎(chǔ)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

E2
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

62.5 pg/mL-1000 pg/mL
靈敏度：

62.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	87
	Range %	84-90
1:4	Average %	87
	Range %	83-91
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of rabbit E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	91-97
EDTA plasma (n=4)	101	98-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.