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Human Interleukin 12,IL-12/P70 ELISA KIT

  • 中文名稱:
    人白介素12(IL-12/P70)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E04599h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人白介素12(IL-12/P70)酶聯(lián)免疫試劑盒(CSB-E04599h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的IL-12/P70含量。IL-12/P70作為重要靶點(diǎn),在免疫調(diào)節(jié)中扮演關(guān)鍵角色。它是一種細(xì)胞因子,能激活T細(xì)胞和NK細(xì)胞,增強(qiáng)機(jī)體免疫反應(yīng)。相關(guān)研究旨在通過調(diào)控其表達(dá)或阻斷其信號傳導(dǎo)通路,用于治療炎癥性疾病、腫瘤等,為開發(fā)新療法提供方向。試劑盒檢測范圍為31.25 pg/ml- 2000pg/ml,靈敏度為7.8 pg/ml。適用于免疫調(diào)節(jié)機(jī)制研究、炎癥性疾病動(dòng)物模型分析、腫瘤微環(huán)境中細(xì)胞因子表達(dá)監(jiān)測等科研領(lǐng)域,特別在評估病原體感染、自身免疫疾病或藥物干預(yù)后IL-12/P70動(dòng)態(tài)變化的研究中具有重要應(yīng)用價(jià)值,為探索Th1型免疫反應(yīng)調(diào)控及免疫治療策略開發(fā)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IL-12/P70
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell culture supernates
  • 檢測范圍:
    31.25 pg/ml - 2000pg/ml
  • 靈敏度:
    7.8 pg/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 本試劑盒所含材料:
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    IL-12/P70是一種細(xì)胞因子,可以促進(jìn)免疫細(xì)胞的增殖和活化,發(fā)揮重要的免疫調(diào)節(jié)作用。最新的研究表明,IL-12/P70在許多自身免疫疾病和腫瘤免疫治療中起著重要的作用。例如,在結(jié)直腸癌治療中,IL-12/P70可以增強(qiáng)免疫反應(yīng),提高患者的治療效果。此外,研究人員也在探索利用IL-12/P70進(jìn)行腫瘤免疫治療的新方法,例如將其與其他免疫因子或化療藥物結(jié)合使用,以提高治療效果。