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Human Lipopolysaccharides,LPS ELISA Kit

  • 中文名稱:
    人脂多糖/內(nèi)毒素(LPS)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09945h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人脂多糖/內(nèi)毒素(LPS)酶聯(lián)免疫試劑盒(CSB-E09945h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的LPS含量。LPS即脂多糖,是革蘭氏陰性菌細(xì)胞壁成分。它可激活機(jī)體免疫反應(yīng),啟動(dòng)炎癥信號(hào)通路,在免疫調(diào)節(jié)、疾病發(fā)生發(fā)展中扮演重要角色。研究其作用機(jī)制有助于理解炎癥性疾病發(fā)病原理,為開(kāi)發(fā)針對(duì)性治療方案提供依據(jù)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL,適用于研究感染性疾病模型中內(nèi)毒素釋放規(guī)律、炎癥信號(hào)通路激活機(jī)制,或用于藥物開(kāi)發(fā)過(guò)程中化合物對(duì)LPS介導(dǎo)炎癥反應(yīng)的調(diào)控作用評(píng)估,為免疫學(xué)、微生物學(xué)及藥理學(xué)等領(lǐng)域的基礎(chǔ)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    LPS
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    6.25 pg/mL-400 pg/mL
  • 靈敏度:
    1.56 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-94  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 94  
    Range % 89-99  
  • 回收率:
    The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-97  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.809 2.960 2.885 2.720  
    200 2.227 2.366 2.297 2.132  
    100 1.515 1.556 1.536 1.371  
    50 0.789 0.755 0.772 0.607  
    25 0.432 0.470 0.451 0.286  
    12.5 0.303 0.325 0.314 0.149  
    6.25 0.235 0.232 0.234 0.069  
    0 0.164 0.165 0.165    
  • 本試劑盒所含材料:
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

為什么不同的物種有不同的ELISA試劑盒。是因?yàn)長(zhǎng)PS的變化嗎?我可以用人LPS ELISA檢測(cè)小鼠血漿中的LPS嗎?

A:
不建議跨物種檢測(cè)。因?yàn)椴煌锓N基質(zhì)是不同的。

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    LPS是一種脂多糖,存在于細(xì)菌細(xì)胞壁中,可引發(fā)嚴(yán)重的炎癥反應(yīng)。最近的研究表明,LPS可以激活多種免疫細(xì)胞,如單核細(xì)胞和樹(shù)突狀細(xì)胞,并誘導(dǎo)它們釋放多種炎癥介質(zhì)。此外,LPS也被認(rèn)為是引發(fā)多種疾病的重要因素之一,例如感染、腸道炎癥和自身免疫性疾病等。研究人員正在努力開(kāi)發(fā)抑制LPS介導(dǎo)的免疫反應(yīng)的新型治療藥物。