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  4. Rat Lipopolysaccharides(LPS) ELISA Kit

Rat Lipopolysaccharides(LPS) ELISA Kit

  • 中文名稱：
    大鼠脂多糖/內(nèi)毒素（LPS）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14247r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠脂多糖/內(nèi)毒素（LPS）酶聯(lián)免疫試劑盒（CSB-E14247r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LPS含量。LPS即脂多糖，是革蘭氏陰性菌細胞壁成分。其背景上，它在引發(fā)內(nèi)毒素休克等病理過程中作用關(guān)鍵。研究機制方面，LPS可激活免疫細胞表面受體，觸發(fā)一系列信號通路，誘導炎性因子釋放，在感染性疾病等研究中是重要靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，試劑盒適用于實驗室環(huán)境中動物模型的炎癥指標監(jiān)測，可支持細菌感染研究、內(nèi)毒素血癥模型構(gòu)建及抗炎藥物開發(fā)等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    LPS
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %102
    Range %96-107
    1:4Average %98
    Range %90-106
    1:8Average %88
    Range %82-104
  • 回收率：
    The recovery of rat LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9384-100
    EDTA plasma (n=4)104100-108
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    101.987 1.999 1.993 1.819
    51.395 1.357 1.376 1.202
    2.50.887 0.897 0.892 0.718
    1.250.595 0.576 0.586 0.412
    0.6250.391 0.384 0.388 0.214
    0.3120.301 0.324 0.313 0.139
    0.1560.247 0.261 0.254 0.080
    00.173 0.175 0.174 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat LPS antibody.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled LPS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

I am interested in your kit Rat Lipopolysaccharides, LPS ELISA Kit (CSB-E14247r) (24 strip-well).
Taking into consideration the number of standards, how many samples can you analyse? Do you need to do this in duplicate?

A:
Thanks for your inquiry.
CSB-E14247r
For this target, dilution is not needed if you test serum and plasma samples. We validated normal serum before and the test value is ND--0.25ng/ml. If you test samples of other modes, you can refer to the research situation of this mode and judge if pretest is needed or not. Take 24T as an example, if you run single well for the standard and the sample, you can test 16 samples. You can also run duplicated wells to reduce errors during the assay.
Pls let me know if you have any further questions. Thank you.