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  4. Mouse Lipopolysaccharides(LPS) ELISA Kit

Mouse Lipopolysaccharides(LPS) ELISA Kit

  • 中文名稱：
    小鼠脂多糖/內(nèi)毒素（LPS）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13066m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠脂多糖/內(nèi)毒素（LPS）酶聯(lián)免疫試劑盒（CSB-E13066m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LPS含量。LPS即脂多糖，是革蘭氏陰性菌細(xì)胞壁的成分。其作為重要的免疫激活劑，可與免疫細(xì)胞表面受體結(jié)合激活天然免疫反應(yīng)。研究圍繞其引發(fā)炎癥信號通路、致病機(jī)制等展開，對理解感染性疾病、開發(fā)相關(guān)治療策略有重要意義。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于實驗動物內(nèi)毒素血癥評估、細(xì)菌感染模型建立、免疫調(diào)節(jié)研究及抗感染藥物篩選等科研場景。在感染性疾病、膿毒癥及炎癥機(jī)制研究中具有重要科研價值，為炎癥機(jī)制研究、病原體 - 宿主互作分析等基礎(chǔ)科研提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    LPS
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-92  
    1:2 Average % 98  
    Range % 91-105  
    1:4 Average % 102  
    Range % 92-110  
    1:8 Average % 96  
    Range % 86-98  
  • 回收率：
    The recovery of mouse LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 89-98  
    EDTA plasma (n=4) 94 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.424 2.522 2.473 2.406  
    5 2.051 1.955 2.003 1.936  
    2.5 1.345 1.255 1.300 1.233  
    1.25 0.679 0.655 0.667 0.600  
    0.625 0.461 0.444 0.453 0.386  
    0.312 0.257 0.239 0.248 0.181  
    0.156 0.171 0.165 0.168 0.101  
    0 0.069 0.065 0.067 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse LPS antibody.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled LPS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

Hi, i have some questions. i am interested about CSB-E13066m elisa kit.its sample types showed to serum, plasme etc. i want serum ascites type

A:
Thanks for your inquiry.
Serum and serum ascites are 2 different sample types.Serum is more normal,and you can refer the information in the published papers at the bottom of the product page.

If you want to detect serum ascites,we suggest you do pre-testing first.Pls let me know if you have any further questions. Thank you.
Q:

When using Mouse Lipopolysaccharides(LPS) ELISA Kit, can I finally translate the results (ng/ml) into the EU/ml?
what is the EU/ml of the standard provided?

A:
Thanks for your inquiry.The the results (ng/ml) can not be translated into the EU/ml results as there is no reduction formula between them.
This kit is supplied with 2 vial of frozen dry standard and the concentration is 10 ng/mL after dissolution;Pls let me know if you have any further questions. Thank you.
Q:

I?am?curious?what?product?you?would?recommend?to?detect?mouse?lipopolysaccharides?with very?small volumes (less than?10?uL)?

A:
Thanks for your inquiry. The amount is too small, if detected, false negatives may exist.Pls consider it carefully.Pls let me know if you have any further questions. Thank you.
Q:

Please provide the following information regarding Mouse Lipopolysaccharides, LPS ELISA Kit (CSB-E13066m)
Can customer use diluent buffer to dilute their plasma samples?

A:
Thanks for your inquiry.
CSB-E13066m
The reagent system of this kit is very special, and dilution is not needed if you test serum or plasma samples.
If you confirm that dilution is needed after the pretest, we suggest you use sample diluent. About pretest pls refer to: https://www.cusabio.com/m-226.html
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 最新研究進(jìn)展：
    脂多糖（LPS）是細(xì)菌細(xì)胞壁的一種成分，在感染和炎癥中發(fā)揮重要作用。最新研究表明，LPS除了其已知的免疫活性外，還可能具有其他生物學(xué)功能，例如參與代謝調(diào)節(jié)和神經(jīng)保護(hù)等。此外，研究人員正在探索LPS與腸道微生物組和健康之間的關(guān)系，以及利用LPS模型研究多種疾病的發(fā)生機(jī)制和治療策略。