Human Undercarboxylated Osteocalcin (ucOC) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒（CSB-E13132h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的ucOC含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域探索骨代謝疾病機(jī)制、評估藥物或營養(yǎng)干預(yù)對骨轉(zhuǎn)換的影響、建立骨代謝相關(guān)疾病模型等研究場景，例如在體外骨細(xì)胞培養(yǎng)體系中監(jiān)測ucOC分泌動態(tài)，或在動物模型中分析骨骼健康狀況。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ucOC
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	91
	Range %	87-96
1:4	Average %	95
	Range %	90-98
1:8	Average %	94
	Range %	86-99

回收率：

The recovery of human ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	97	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.