Rat Undercarboxylated Osteocalcin (ucOC) ELISA Kit

說(shuō)明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒（CSB-E17798r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ucOC含量。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于多種科研場(chǎng)景，如評(píng)估骨組織工程材料的成骨效果、探究藥物或營(yíng)養(yǎng)干預(yù)對(duì)骨代謝的影響、構(gòu)建骨相關(guān)疾病動(dòng)物模型后的生物標(biāo)志物分析等。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

ucOC
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	87-101
1:2	Average %	101
	Range %	94-105
1:4	Average %	95
	Range %	88-98
1:8	Average %	102
	Range %	94-107

回收率：

The recovery of rat ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	100	94-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.