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  4. Mouse Undercarboxylated Osteocalcin (ucOC) ELISA Kit

Mouse Undercarboxylated Osteocalcin (ucOC) ELISA Kit

  • 中文名稱：
    小鼠羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17681m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒（CSB-E17681m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的ucOC含量。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，為骨生物學(xué)研究提供可靠工具，特別適用于骨質(zhì)疏松動(dòng)物模型構(gòu)建、骨代謝調(diào)控機(jī)制研究、藥物干預(yù)效果評(píng)價(jià)等科研領(lǐng)域，其優(yōu)化的檢測(cè)體系能有效支持血清等復(fù)雜樣本中微量ucOC的穩(wěn)定檢出，為探索骨骼系統(tǒng)疾病發(fā)生發(fā)展機(jī)制提供數(shù)據(jù)支撐。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    ucOC
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %85
    Range %80-90
    1:10Average %92
    Range %86-96
    1:20Average %94
    Range %87-100
    1:40Average %97
    Range %92-101
  • 回收率：
    The recovery of mouse ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-98
    EDTA plasma (n=4)9087-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.475 2.352 2.414 2.237
    52.058 1.976 2.017 1.840
    2.51.646 1.534 1.590 1.413
    1.251.217 1.139 1.178 1.001
    0.6250.821 0.797 0.809 0.632
    0.3120.593 0.579 0.586 0.409
    0.1560.262 0.247 0.255 0.078
    00.184 0.169 0.177 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Hi, I'm interested in purchasing the ELISA unOC ELISA kit .
Do you have or know of any published data demonstrating the ability of your kits to detect undercarboxylated and total osteocalcin levels in mice?

A:
Thanks for your inquiry.
The total osteocalcin you mentioned should include fully carboxylated，undercarboxylated forms and fragments.
Sorry we don't have this kit.
CSB-E17681m This kit detects Undercarboxylated Osteocalcin (ucOC) .
Sorry there is no publication for this kit at present. Maybe customers didn't publish yet.