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  4. Human adrenocorticotropic hormone (ACTH) ELISA kit

Human adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱：
    人促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06873h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-E06873h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACTH含量。ACTH即促腎上腺皮質(zhì)激素，由腺垂體分泌。其背景在于它可促進(jìn)腎上腺皮質(zhì)增生、合成與分泌腎上腺皮質(zhì)激素。研究機(jī)制方面，它與腎上腺皮質(zhì)細(xì)胞膜上受體結(jié)合，激活腺苷酸環(huán)化酶-cAMP-蛋白激酶系統(tǒng)，從而調(diào)節(jié)腎上腺皮質(zhì)激素合成與分泌。試劑盒檢測(cè)范圍為12.5 pg/mL-200 pg/mL，適用于科研領(lǐng)域中對(duì)下丘腦 - 垂體 - 腎上腺軸功能的機(jī)制研究、疾病模型構(gòu)建以及藥物干預(yù)效果的評(píng)估；為研究ACTH在生理病理過程中的動(dòng)態(tài)變化提供可靠工具，尤其適用于神經(jīng)內(nèi)分泌學(xué)、代謝性疾病及應(yīng)激反應(yīng)相關(guān)的基礎(chǔ)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    ACTH
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    12.5 pg/mL-200 pg/mL
  • 靈敏度：
    12.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-93  
    1:2 Average % 94  
    Range % 89-99  
    1:4 Average % 95  
    Range % 89-99  
    1:8 Average % 92  
    Range % 87-96  
  • 回收率：
    The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 97 95-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.192 0.187 0.190    
    100 0.337 0.328 0.333    
    50 0.601 0.613 0.607    
    25 1.127 1.140 1.134    
    12.5 2.316 2.212 2.264    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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