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  4. Human advanced glycation end products,AGEs ELISA Kit

Human advanced glycation end products,AGEs ELISA Kit

  • 中文名稱：
    人晚期糖基化終末產(chǎn)物(AGEs)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09412h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人晚期糖基化終末產(chǎn)物(AGEs)酶聯(lián)免疫試劑盒（CSB-E09412h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的AGEs含量。AGEs即晚期糖基化終末產(chǎn)物，在高糖環(huán)境等條件下大量生成并在體內(nèi)蓄積。其背景與糖尿病、衰老等多種病理過(guò)程相關(guān)。研究機(jī)制上，AGEs可與受體相互作用，激活多種信號(hào)通路，引發(fā)氧化應(yīng)激和炎癥反應(yīng)，導(dǎo)致組織損傷和功能障礙。試劑盒檢測(cè)范圍為0.78 μg/mL-50 μg/mL，本試劑盒基于特異性抗體對(duì)捕獲和檢測(cè)靶標(biāo)，適用于科研場(chǎng)景中AGEs相關(guān)機(jī)制研究，例如糖尿病模型動(dòng)物血清/組織樣本分析、體外藥物干預(yù)對(duì)AGEs生成抑制效果的評(píng)估，或阿爾茨海默病等神經(jīng)退行性疾病中AGEs沉積的病理學(xué)關(guān)聯(lián)探索。支持科研人員高效開(kāi)展AGEs在代謝性疾病、慢性炎癥或衰老機(jī)制中的基礎(chǔ)研究與體外實(shí)驗(yàn)數(shù)據(jù)獲取。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫：
    AGEs
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.78 μg/mL-50 μg/mL
  • 靈敏度：
    0.195 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-89
    1:2Average %90
    Range %85-95
    1:4Average %94
    Range %90-99
    1:8Average %104
    Range %100-109
  • 回收率：
    The recovery of human AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-96
    EDTA plasma (n=4)9792-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/mlOD1OD2AverageCorrected
    502.698 2.652 2.675 2.534
    252.076 2.082 2.079 1.938
    12.51.204 1.283 1.244 1.103
    6.250.639 0.669 0.654 0.513
    3.120.388 0.374 0.381 0.240
    1.560.261 0.259 0.260 0.119
    0.780.187 0.179 0.183 0.042
    00.145 0.137 0.141 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human AGEs antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled AGEs antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the chromogenic reaction.
    • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

I would like to know if this kit can evaluate all AGEs or only a specific one, like arboximetilisina.

A:
Thanks for your inquiry.
This kit quantifys all AGEs.