Mouse advanced glycation end products,AGEs ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠晚期糖基化終末產(chǎn)物(AGEs)酶聯(lián)免疫試劑盒（CSB-E09414m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的AGEs含量。AGEs是晚期糖基化終末產(chǎn)物。背景上，它是糖與蛋白質(zhì)等非酶促反應(yīng)的產(chǎn)物，在糖尿病、衰老等過程中積累。研究機(jī)制表明，其通過與受體結(jié)合，激活多條信號(hào)通路，引發(fā)氧化應(yīng)激、炎癥反應(yīng)等，參與糖尿病并發(fā)癥等多種疾病發(fā)生發(fā)展。試劑盒檢測(cè)范圍為0.156 μg/mL-10 μg/mL，科研人員可借助探究AGEs在代謝性疾病模型中的動(dòng)態(tài)變化、評(píng)估抗氧化或抗糖化藥物的干預(yù)效果，以及衰老相關(guān)分子機(jī)制研究，為生物醫(yī)學(xué)領(lǐng)域提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AGEs
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	97
	Range %	92-103
1:4	Average %	99
	Range %	94-110
1:8	Average %	95
	Range %	87-100

回收率：

The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-99
EDTA plasma (n=4)	97	92-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














µg/ml	OD1	OD2	Average	Corrected
10	1.958	1.900	1.929	1.779
5	1.583	1.602	1.593	1.443
2.5	1.160	1.154	1.157	1.007
1.25	0.754	0.739	0.747	0.597
0.625	0.471	0.434	0.453	0.303
0.312	0.342	0.319	0.331	0.181
0.156	0.253	0.239	0.246	0.096
0	0.152	0.148	0.150