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  4. Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

  • 中文名稱：
    人血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04493h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E04493h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的ANG-Ⅱ含量。ANG-Ⅱ即血管緊張素Ⅱ，在心血管系統(tǒng)調(diào)節(jié)中有著重要背景。它可引起血管收縮、醛固酮釋放等。研究機(jī)制方面，其通過與特異性受體結(jié)合，激活多條信號通路，影響細(xì)胞生長、增殖，在高血壓、心衰等疾病發(fā)病中扮演關(guān)鍵角色。試劑盒檢測范圍為31.25 pg/ml-2000 pg/ml.，適用于科研場景中探究ANG - Ⅱ在心血管疾病模型中的動態(tài)變化、藥物干預(yù)效果評估或相關(guān)代謝調(diào)控機(jī)制分析，為心血管疾病基礎(chǔ)研究、藥理學(xué)實驗及生物標(biāo)志物探索提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    angiotensin Ⅱ, angiotensin 2, ANG2
  • 縮寫：
    ANG-Ⅱ
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    31.25 pg/ml-2000 pg/ml.
  • 靈敏度：
    15.6 pg/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ANG- Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    85-101

    1:2

    Average %

    89

    Range %

    82-95

    1:4

    Average %

    93

    Range %

    88-99

    1:8

    Average %

    96

    Range %

    89-103

  • 回收率：

    The recovery of human ANG- Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    84-98

    EDTA plasma (n=4)

    87

    81-95

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    2000

    0.159

    0.154

    0.157

    1000

    0.214

    0.222

    0.218

    500

    0.292

    0.286

    0.289

    250

    0.370

    0.398

    0.384

    125

    0.568

    0.561

    0.565

    62.5

    0.915

    0.933

    0.924

    31.25

    1.346

    1.284

    1.315

    0

    2.207

    2.125

    2.166

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANG-II antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled ANG-II antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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