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  4. Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

  • 中文名稱：
    人1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12636h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒（CSB-E12636h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IP3含量。IP3即三磷酸肌醇，是細胞內重要的第二信使。其背景在于它在細胞信號轉導中扮演關鍵角色。研究機制上，當細胞外信號激活相應受體，通過G蛋白激活磷脂酶C，水解磷脂酰肌醇生成IP3，它能促使內質網釋放Ca2?，引發(fā)一系列細胞反應。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，尤其適用于體外研究細胞信號通路激活程度、藥物靶點作用機制或疾病模型中IP3動態(tài)變化的分析；為探究G蛋白偶聯(lián)受體介導的磷酸肌醇代謝、細胞應激反應調控等基礎研究提供可靠工具，廣泛適配于神經生物學、藥理學及病理生理學等領域的科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    IP3
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    5 pg/mL-1000 pg/mL
  • 靈敏度：
    2.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-105  
    1:2 Average % 94  
    Range % 89-100  
    1:4 Average % 94  
    Range % 87-102  
    1:8 Average % 95  
    Range % 90-102  
  • 回收率：
    The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-100  
    EDTA plasma (n=4) 92 87-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.202 0.211 0.207    
    250 0.350 0.349 0.350    
    80 0.600 0.594 0.597    
    20 1.029 1.018 1.024    
    5 1.326 1.345 1.336    
    0 2.102 2.130 2.116    
  • 數據處理：
  • 貨期：
    3-5 working days

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相關問答

■ 常見問題解答
Q:

I would like to receive more information about this assay, particularly regarding specificity for IP3.
Please provide some more information about the following.
a. capture antibody or capture antigen
b. Detection antibody (if applicable)
c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
Are you aware of any product specific references for this assay? If yes please can you provide links to these references. If you have any other information about validation of the antibodies in other applications e.g. WB this information would be helpful also.

A:
Thanks for your inquiry.
CSB-E12636h
This is a competitive kit.
The plate is coated with goat-anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody.
The standard is purified IP3.
The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
Pls let me know if you have any further questions. Thank you.