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  4. Rat inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Rat inositol 1,4,5,-trisphosphate(IP3) ELISA kit

  • 中文名稱：
    大鼠1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒　
  • 貨號：
    CSB-E13004r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒　（CSB-E13004r）為競爭法ELISA試劑盒，定量檢測血清、血漿、tissue 勻漿、組織培養(yǎng)上清液、細胞裂解物樣本中的IP3含量。IP3（肌醇三磷酸）是一種重要的細胞信號分子，由磷脂酶C（PLC）水解磷脂酰肌醇-4,5-二磷酸（PIP2）產(chǎn)生。它作為第二信使，通過結(jié)合內(nèi)質(zhì)網(wǎng)上的IP3受體，觸發(fā)鈣離子釋放，從而調(diào)節(jié)細胞內(nèi)鈣信號，影響多種細胞反應，如平滑肌收縮、血小板聚集等。研究IP3信號轉(zhuǎn)導機制有助于理解相關(guān)疾病的發(fā)生和發(fā)展，為疾病治療提供新靶點。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，該產(chǎn)品適用于神經(jīng)生物學、心血管疾病模型、藥物作用機制等研究領(lǐng)域，可用于探索細胞內(nèi)信號通路、評估化合物干預效果。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IP3
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates, cell lysates
  • 檢測范圍：
    5 pg/mL-1000 pg/mL
  • 靈敏度：
    2.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-96  
    1:2 Average % 94  
    Range % 89-98  
    1:4 Average % 96  
    Range % 87-102  
    1:8 Average % 97  
    Range % 91-104  
  • 回收率：
    The recovery of rat IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 91-105  
    EDTA plasma (n=4) 95 92-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    1000 0.141 0.143 0.142 　  
    250 0.270 0.261 0.266 　  
    80 0.423 0.440 0.432 　  
    20 0.744 0.759 0.752 　  
    5 1.156 1.162 1.159 　  
    0 2.199 2.214 2.207 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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