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  4. Mouse inositol 1,4,5,-trisphosphate(IP3) ELISA Kit

Mouse inositol 1,4,5,-trisphosphate(IP3) ELISA Kit

  • 中文名稱：
    小鼠1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13410m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒（CSB-E13410m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的IP3含量。IP3（肌醇三磷酸）是一種重要的細(xì)胞信號(hào)分子，參與G蛋白偶聯(lián)受體介導(dǎo)的信號(hào)轉(zhuǎn)導(dǎo)。它由PLC水解PIP2產(chǎn)生，能結(jié)合內(nèi)質(zhì)網(wǎng)上的IP3受體，引發(fā)鈣離子釋放，進(jìn)而調(diào)控細(xì)胞內(nèi)多種生理過(guò)程。研究IP3信號(hào)通路有助于理解細(xì)胞內(nèi)鈣信號(hào)調(diào)控機(jī)制及其在疾病發(fā)生中的作用。試劑盒檢測(cè)范圍為0.78 pg/mL-50 pg/mL，適用于實(shí)驗(yàn)室研究細(xì)胞信號(hào)通路激活、受體功能評(píng)估或藥物干預(yù)后IP3動(dòng)態(tài)變化。為探索IP3在生理病理機(jī)制中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    IP3
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %86
    Range %82-95
    1:200Average %95
    Range %91-99
    1:400Average %97
    Range %96-105
    1:800Average %98
    Range %89-102
  • 回收率：
    The recovery of mouse IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10498-107
    EDTA plasma (n=4)9892-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    502.695 2.594 2.645 2.454
    252.004 1.903 1.954 1.763
    12.51.111 1.204 1.158 0.967
    6.250.681 0.670 0.676 0.485
    3.120.441 0.452 0.447 0.256
    1.560.325 0.315 0.320 0.129
    0.780.260 0.249 0.255 0.064
    00.191 0.190 0.191 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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