Human plasmin-antiplasmin complex,PAP ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人纖溶酶抗纖溶酶復(fù)合物(PAP)酶聯(lián)免疫試劑盒（CSB-E08434h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液樣本中的PAP含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品可為研究血栓形成與溶解的動態(tài)平衡、評估抗凝藥物作用機(jī)制或探索纖溶相關(guān)疾病的生物標(biāo)志物提供可靠工具，例如在心血管疾病模型、腫瘤微環(huán)境分析或炎癥相關(guān)實驗中進(jìn)行樣本內(nèi)源性PAP水平的精準(zhǔn)測定。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

PAP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	80-92
1:200	Average %	100
	Range %	91-108
1:400	Average %	100
	Range %	92-109
1:800	Average %	92
	Range %	86-100

回收率：

The recovery of human PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-101
EDTA plasma (n=4)	93	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.