Mouse plasmin-antiplasmin complex,PAP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠纖溶酶抗纖溶酶復(fù)合物(PAP)酶聯(lián)免疫試劑盒（CSB-E08436m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PAP含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于體外實驗環(huán)境下檢測小鼠模型中與血栓形成、溶栓治療或炎癥反應(yīng)相關(guān)的PAP動態(tài)變化，例如在血栓性疾病模型評估、抗凝藥物作用機(jī)制研究或纖溶系統(tǒng)調(diào)控實驗中定量分析樣本生物標(biāo)志物水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

PAP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	83-94
1:10	Average %	97
	Range %	92-102
1:20	Average %	105
	Range %	98-111
1:40	Average %	93
	Range %	87-99

回收率：

The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	101	95-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.