Rat plasmin-antiplasmin complex,PAP ELISA Kit
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中文名稱(chēng):大鼠纖溶酶抗纖溶酶復(fù)合物(PAP)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08435r
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠纖溶酶抗纖溶酶復(fù)合物(PAP)酶聯(lián)免疫試劑盒(CSB-E08435r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、組織勻漿樣本中的PAP含量。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL,適用于心血管疾病模型研究、凝血 - 纖溶平衡機(jī)制探索或藥物干預(yù)效果評(píng)估等科研領(lǐng)域。可為實(shí)驗(yàn)室提供穩(wěn)定可靠的PAP定量數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:N/A
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縮寫(xiě):PAP
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種屬:Rattus norvegicus (Rat)
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樣本類(lèi)型:serum, tissue homogenates
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檢測(cè)范圍:7.8 ng/mL-500 ng/mL
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靈敏度:1.95 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 90-103 1:2 Average % 98 Range % 91-105 1:4 Average % 99 Range % 94-106 1:8 Average % 105 Range % 100-110 -
回收率:
The recovery of rat PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 94-106 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 500 1.997 2.097 2.047 1.886 250 1.468 1.568 1.518 1.357 125 0.957 1.007 0.982 0.821 62.5 0.627 0.637 0.632 0.471 31.2 0.390 0.400 0.395 0.234 15.6 0.291 0.301 0.296 0.135 7.8 0.242 0.247 0.245 0.084 0 0.160 0.162 0.161 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Massive Amounts of Tissue Factor Induce Fibrinogenolysis Without Tissue Hypoperfusion in Rats Hayakawa M et al,Shock.?,2013
- Long??erm estrogen treatment of mice with a prothrombotic phenotype induces sustained increases in thrombin generation without affecting tissue fibrin deposition Cleuren AC et al,J Thromb Haemost,2012
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