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  4. Human prothrombin fragment 1+2,F1+2 ELISA kit

Human prothrombin fragment 1+2,F1+2 ELISA kit

  • 中文名稱：
    人凝血酶原片段F1+2(F1+2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09822h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人凝血酶原片段F1+2(F1+2)酶聯(lián)免疫試劑盒（CSB-E09822h）檢測血清、血漿、組織勻漿樣本中的F1+2含量。人凝血酶原片段F1+2是凝血酶原激活轉化為凝血酶過程中釋放的穩(wěn)定降解產物，其含量反映凝血酶早期激活水平，可用于診斷血栓前狀態(tài)和血栓性疾病。研究顯示，F(xiàn)1+2在凝血途徑中起關鍵作用，其檢測有助于疾病診斷和治療監(jiān)測。試劑盒檢測范圍為0.03 nmol/L -2 nmol/L，該產品廣泛應用于基礎醫(yī)學研究中凝血/纖溶系統(tǒng)動態(tài)評估、藥物抗凝效果評價、動物模型血栓形成機制探索等科研領域，為心血管疾病、腫瘤轉移等病理過程的分子機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 縮寫：
    F1+2
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.03 nmol/L -2 nmol/L
  • 靈敏度：
    0.007 nmol/L
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    89-99

    1:2

    Average %

    98

    Range %

    93-102

    1:4

    Average %

    86

    Range %

    81-93

    1:8

    Average %

    92

    Range %

    88-97

  • 回收率：

    The recovery of human F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    90-96

    EDTA plasma (n=4)

    87

    83-90

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    nmol/L

    OD1

    OD2

    Average

    Corrected

    2

    2.296

    2.274

    2.285

    2.137

    1

    1.445

    1.498

    1.472

    1.324

    0.5

    0.916

    0.936

    0.926

    0.778

    0.25

    0.538

    0.568

    0.553

    0.405

    0.125

    0.376

    0.398

    0.387

    0.239

    0.06

    0.270

    0.291

    0.281

    0.133

    0.03

    0.218

    0.216

    0.217

    0.069

    0

    0.146

    0.149

    0.148

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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