Rabbit prothrombin fragment 1+2 (F1+2) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔凝血酶原片段F1+2(F1+2)酶聯(lián)免疫試劑盒（CSB-EQ027672RB）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的F1+2含量。兔凝血酶原片段F1+2（F1+2）是凝血酶原分子的氨基末端片段，當(dāng)凝血酶被激活時(shí)釋放，具有多肽特性，半衰期約90分鐘。其在凝血過程中通過檢測(cè)F1+2水平，可反映凝血酶的活性，是評(píng)估凝血功能的重要指標(biāo)。試劑盒檢測(cè)范圍為0.312 pmol/mL-20 pmol/mL，常用于血栓形成機(jī)制、抗凝藥物作用評(píng)估等研究；支持科研人員探究心血管疾病模型、凝血功能障礙動(dòng)物實(shí)驗(yàn)中的F1+2動(dòng)態(tài)變化；為凝血與纖溶系統(tǒng)調(diào)控、藥物干預(yù)效果分析等基礎(chǔ)研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

F1+2
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 pmol/mL-20 pmol/mL
靈敏度：

0.078 pmol/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	98
	Range %	92-104
1:4	Average %	97
	Range %	93-101
1:8	Average %	87
	Range %	82-95

回收率：

The recovery of rabbit F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-92
EDTA plasma (n=4)	92	85-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.