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  4. Mouse C-Peptide ELISA Kit

Mouse C-Peptide ELISA Kit

  • 中文名稱：
    小鼠C肽(C-Peptide)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05068m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠C肽(C-Peptide)酶聯(lián)免疫試劑盒（CSB-E05068m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的C-Peptide含量。C-Peptide即C肽，是胰島素原裂解產(chǎn)生的與胰島素等分子的肽類物質(zhì)。研究發(fā)現(xiàn)它有獨(dú)立于胰島素的生物學(xué)作用，可改善神經(jīng)、腎臟等器官的血流，調(diào)節(jié)細(xì)胞內(nèi)信號(hào)傳導(dǎo)，其機(jī)制為結(jié)合特定細(xì)胞膜受體，激活下游信號(hào)通路，正被深入研究以挖掘更多臨床價(jià)值。試劑盒檢測(cè)范圍為2.86 ng/mL-50 ng/mL，糖尿病模型研究、胰島素分泌機(jī)制探索及代謝調(diào)控實(shí)驗(yàn)、胰島素相關(guān)代謝研究、糖尿病動(dòng)物模型評(píng)估以及藥物干預(yù)效果分析、科研領(lǐng)域?qū)Ζ录?xì)胞功能動(dòng)態(tài)監(jiān)測(cè)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    C-Peptide
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    3.125 ng/ml-50 ng/ml
  • 靈敏度：
    0.625 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 85-99  
    1:2 Average % 90  
    Range % 85-96  
    1:4 Average % 95  
    Range % 87-106  
    1:8 Average % 95  
    Range % 89-100  
  • 回收率：
    The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-100  
    EDTA plasma (n=4) 95 91-103  
                 
                 
  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse C-peptide antibody.
      • Six vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial HRP-conjugated C-peptide antibody(6 ml/bottle) ---Bind to the C-peptide in the samples or standards and react with the substrate to make the solution chromogenic.
      • One vial Wash Buffer (20x concentrate) (15ml/bottle) ---Wash away unbound or free substances.
      • Substrate A (1 x 7 ml)
      • Substrate B (1 x 7 ml)
      • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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