Rat C-Peptide ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠C肽(C-Peptide)酶聯(lián)免疫試劑盒（CSB-E05067r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C-Peptide含量。C-Peptide即C肽，是胰島素原裂解產(chǎn)生的等分子肽類片段。它可反映內(nèi)源性胰島素分泌情況。研究機(jī)制上，C肽具有改善神經(jīng)傳導(dǎo)、增加腎血流量等作用，其與糖尿病并發(fā)癥、腎臟疾病等的關(guān)系是研究熱點(diǎn)。試劑盒檢測范圍為1.25 ng/mL-20 ng/mL，作為評估胰島β細(xì)胞功能的重要生物標(biāo)志物，廣泛應(yīng)用于糖尿病發(fā)病機(jī)制、胰島功能調(diào)控及相關(guān)藥物干預(yù)研究領(lǐng)域。適用于評估實(shí)驗(yàn)動物模型中的胰島素分泌動態(tài)、胰腺組織功能狀態(tài)及代謝相關(guān)研究。為糖尿病研究、藥物作用評價(jià)及代謝調(diào)控機(jī)制探索提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C-Peptide
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 ng/mL-20 ng/mL
靈敏度：

1.25 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C-peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-103
1:2	Average %	90
	Range %	85-94
1:4	Average %	84
	Range %	80-88
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat C-peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	96	91-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.