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  4. Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名稱(chēng)：
    小鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E10787m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯(lián)免疫試劑盒（CSB-E10787m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Aβ1-42含量。Aβ1-42是重要的阿爾茨海默病相關(guān)靶點(diǎn)。在患者腦內(nèi)該蛋白異常聚集形成淀粉樣斑塊，破壞神經(jīng)細(xì)胞功能與結(jié)構(gòu)。研究聚焦其產(chǎn)生、聚集過(guò)程及與神經(jīng)毒性的關(guān)聯(lián)，旨在通過(guò)抑制生成、促進(jìn)清除等機(jī)制來(lái)改善病情、研發(fā)治療藥物。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于阿爾茨海默病小鼠模型構(gòu)建、Aβ病理機(jī)制研究、藥物干預(yù)效果評(píng)估等實(shí)驗(yàn)場(chǎng)景，為神經(jīng)科學(xué)領(lǐng)域提供可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 縮寫(xiě)：
    Aβ1-42
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-99  
    1:2 Average % 95  
    Range % 85-105  
    1:4 Average % 96  
    Range % 90-105  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率：
    The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 93 85-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.490 2.451 2.471 2.337  
    500 2.049 1.985 2.017 1.883  
    250 1.357 1.385 1.371 1.237  
    125 0.866 0.837 0.852 0.718  
    62.5 0.517 0.524 0.521 0.387  
    31.2 0.347 0.355 0.351 0.217  
    15.6 0.244 0.228 0.236 0.102  
    0 0.135 0.132 0.134 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a 96T sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    Aβ1-42是β淀粉樣蛋白（Aβ）家族的一員，它是阿爾茨海默?。ˋD）病理學(xué)的主要成分。最近的研究表明，Aβ1-42的積聚是導(dǎo)致AD的主要因素之一。已經(jīng)發(fā)現(xiàn)許多抗Aβ1-42藥物，如免疫療法和β-淀粉樣蛋白藥物，這些藥物能夠清除Aβ1-42，并恢復(fù)AD患者的認(rèn)知能力。此外，一些研究還發(fā)現(xiàn)，Aβ1-42可能是癲癇的重要病理因素，這為癲癇治療提供了新的思路。