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Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名稱(chēng):
    大鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E10786r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯(lián)免疫試劑盒(CSB-E10786r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的Aβ1-42含量。Aβ1-42是重要的阿爾茨海默病相關(guān)靶點(diǎn)。其在腦內(nèi)異常聚集形成淀粉樣斑塊,被認(rèn)為是該病發(fā)病的關(guān)鍵病理特征。研究聚焦于其生成、清除機(jī)制、神經(jīng)毒性作用等,期望通過(guò)干預(yù)Aβ1-42代謝過(guò)程開(kāi)發(fā)有效治療手段。試劑盒檢測(cè)范圍為0.9 pg/mL-60 pg/mL,適用于神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究,如阿爾茨海默病動(dòng)物模型中Aβ1 - 42的動(dòng)態(tài)變化觀(guān)察、藥物干預(yù)效果評(píng)價(jià)或病理機(jī)制探索,其寬泛的樣本兼容性可滿(mǎn)足腦組織、血液等多種生物樣本的檢測(cè)需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 縮寫(xiě):
    Aβ1-42
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.9 pg/mL-60 pg/mL
  • 靈敏度:
    0.225 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %83-90
    1:2Average %94
    Range %90-98
    1:4Average %101
    Range %97-104
    1:8Average %96
    Range %93-99
  • 回收率:
    The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10095-105
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    602.281 2.274 2.278 2.136
    301.599 1.576 1.588 1.446
    150.967 0.924 0.946 0.804
    7.50.538 0.528 0.533 0.391
    3.750.395 0.371 0.383 0.241
    1.80.321 0.312 0.317 0.175
    0.90.235 0.250 0.243 0.101
    00.141 0.143 0.142  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

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相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

我們的實(shí)驗(yàn)室打算購(gòu)買(mǎi)β淀粉樣蛋白1-42,Aβ1-42 ELISA試劑盒,貨號(hào)是CSB-E10786r。但是,我們使用的是含有SDS的RIPA緩沖液來(lái)裂解組織,同時(shí)我們也用相同的RIPA緩沖液和1x PBS稀釋了樣品。我想知道這樣做是否會(huì)影響試劑盒的效果

A:
對(duì)于ELISA檢測(cè),一般不建議使用含有SDS的緩沖液處理樣品。這是因?yàn)镾DS可以破壞分子與分子間的氫鍵,即破壞蛋白質(zhì)的二級(jí)和三級(jí)結(jié)構(gòu),并可能影響ELISA的檢測(cè)。由于您的樣品已經(jīng)被處理過(guò),我們只能建議您先使用處理后的樣品進(jìn)行梯度預(yù)實(shí)驗(yàn)。我們以前沒(méi)有這樣做過(guò),所以我們只能建議你試一試。