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  4. Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

  • 中文名稱：
    小鼠血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04495m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E04495m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的ANG-Ⅱ含量。ANG-Ⅱ即血管緊張素Ⅱ，在人體血壓調節(jié)、體液平衡等生理過程中扮演關鍵角色。其過度激活與高血壓、心血管疾病等多種病癥相關。研究主要聚焦于其信號傳導通路、與受體相互作用機制，為研發(fā)針對性藥物以防治相關疾病提供理論依據(jù)。試劑盒檢測范圍為15.6 pg/ml-1000 pg/ml.，適用于心血管疾病動物模型研究、降壓藥物作用機制探索、內分泌調控實驗等科研領域，為小鼠血清或血漿樣本中ANG - Ⅱ的動態(tài)監(jiān)測提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    angiotensin Ⅱ, angiotensin 2, ANG2
  • 縮寫：
    ANG-Ⅱ
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 pg/ml-1000 pg/ml.
  • 靈敏度：
    7.8 pg/ml.
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    88

    Range %

    83-94

    1:2

    Average %

    91

    Range %

    85-96

    1:4

    Average %

    90

    Range %

    84-98

    1:8

    Average %

    93

    Range %

    86-99

  • 回收率：

    The recovery of mouse ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    84-98

    EDTA plasma (n=4)

    87

    81-95

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    1000

    0.161

    0.166

    0.164

    500

    0.185

    0.192

    0.189

    250

    0.228

    0.237

    0.233

    125

    0.334

    0.324

    0.329

    62.5

    0.568

    0.576

    0.572

    31.25

    0.902

    0.891

    0.897

    15.6

    1.208

    1.217

    1.212

    0

    2.176

    2.223

    2.199

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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