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Mouse thrombin-antithrombin complex,TAT ELISA Kit

  • 中文名稱:
    小鼠凝血酶抗凝血酶復合物(TAT)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08433m
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠凝血酶抗凝血酶復合物(TAT)酶聯(lián)免疫試劑盒(CSB-E08433m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TAT含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,常用于評估凝血激活狀態(tài)及相關(guān)病理機制研究;適用于小鼠模型的血栓性疾病、彌散性血管內(nèi)凝血(DIC)等凝血功能障礙研究,或藥物干預(yù)下凝血/抗凝血系統(tǒng)調(diào)控機制的體外實驗分析;為心血管疾病、炎癥反應(yīng)或腫瘤相關(guān)凝血異常的動物模型研究提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    TAT
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10 Average % 87  
    Range % 82-93  
    1:20 Average % 95  
    Range % 90-101  
    1:40 Average % 105  
    Range % 96-112  
    1:80 Average % 93  
    Range % 87-98  
  • 回收率:
    The recovery of mouse TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 93-100  
    EDTA plasma (n=4) 90 85-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.658 2.456 2.557 2.374  
    500 2.214 2.113 2.164 1.981  
    250 1.503 1.522 1.513 1.330  
    125 0.855 0.904 0.880 0.697  
    62.5 0.542 0.563 0.553 0.370  
    31.2 0.413 0.435 0.424 0.241  
    15.6 0.291 0.302 0.297 0.114  
    0 0.188 0.178 0.183    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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