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  3. 酶聯(lián)免疫試劑盒
  4. Rat thrombin-antithrombin complex,TAT ELISA Kit

Rat thrombin-antithrombin complex,TAT ELISA Kit

  • 中文名稱(chēng)：
    大鼠凝血酶抗凝血酶復(fù)合物(TAT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08432r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠凝血酶抗凝血酶復(fù)合物(TAT)酶聯(lián)免疫試劑盒（CSB-E08432r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的TAT含量。大鼠凝血酶抗凝血酶復(fù)合物（TAT）是凝血酶與抗凝血酶結(jié)合形成的復(fù)合物，反映凝血酶生成和活性，用于評(píng)估抗凝藥物效果、研究血栓形成機(jī)制及血液疾病診斷。通過(guò)ELISA技術(shù)檢測(cè)TAT水平，可監(jiān)測(cè)機(jī)體凝血狀態(tài)和血栓前狀態(tài)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研場(chǎng)景中動(dòng)態(tài)監(jiān)測(cè)凝血激活狀態(tài)，如在心血管疾病模型、血栓性疾病動(dòng)物實(shí)驗(yàn)或藥物干預(yù)研究中分析凝血與抗凝血平衡，也可用于體外細(xì)胞實(shí)驗(yàn)中探究凝血相關(guān)信號(hào)通路或藥物對(duì)凝血級(jí)聯(lián)反應(yīng)的影響。其寬泛的樣本兼容性支持從基礎(chǔ)研究到藥物開(kāi)發(fā)的多層次實(shí)驗(yàn)需求，為凝血功能相關(guān)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    TAT
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %97
    Range %91-105
    1:10Average %103
    Range %97-109
    1:20Average %95
    Range %90-98
    1:40Average %96
    Range %90-102
  • 回收率：
    The recovery of rat TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8882-94
    EDTA plasma (n=4)9487-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.504 2.385 2.445 2.324
    10001.712 1.633 1.673 1.552
    5001.010 1.001 1.006 0.885
    2500.606 0.625 0.616 0.495
    1250.387 0.396 0.392 0.271
    62.50.289 0.295 0.292 0.171
    31.250.193 0.196 0.195 0.074
    00.120 0.121 0.121 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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