Pig Platelet-Derived Growth Factor AB (PDGF-AB) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬血小板衍生生長因子AB(PDGF-AB)酶聯(lián)免疫試劑盒（CSB-EQ027978PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-AB含量。PDGF - AB是一種血小板衍生生長因子，在細(xì)胞生長、增殖和分化等過程中起重要作用。其異常表達(dá)與腫瘤、纖維化等疾病的發(fā)生發(fā)展相關(guān)。研究機(jī)制聚焦于其信號(hào)通路，通過干預(yù)該信號(hào)通路有望為相關(guān)疾病的治療提供新策略。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于豬模型研究中PDGF - AB的動(dòng)態(tài)監(jiān)測，例如在創(chuàng)傷修復(fù)、心血管疾病機(jī)制或干細(xì)胞分化等基礎(chǔ)科研領(lǐng)域，為探索生長因子網(wǎng)絡(luò)調(diào)控提供有效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-AB
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	90
	Range %	85-99
1:4	Average %	93
	Range %	84-97
1:8	Average %	95
	Range %	83-100

回收率：

The recovery of pig PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-101
EDTA plasma (n=4)	93	87-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.