Rat Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
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中文名稱:大鼠血小板衍生生長(zhǎng)因子AB(PDGF-AB)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04703r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠血小板衍生生長(zhǎng)因子AB(PDGF-AB)酶聯(lián)免疫試劑盒(CSB-E04703r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PDGF-AB含量。PDGF - AB是一種重要的靶點(diǎn)。它屬于血小板衍生生長(zhǎng)因子家族,在細(xì)胞生長(zhǎng)、增殖、遷移和血管生成等過(guò)程中有關(guān)鍵作用。目前研究聚焦于其信號(hào)通路機(jī)制,探索通過(guò)阻斷該靶點(diǎn)來(lái)抑制腫瘤生長(zhǎng)、纖維化等疾病發(fā)展,為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL,特別適用于大鼠模型研究中PDGF - AB的動(dòng)態(tài)監(jiān)測(cè),例如創(chuàng)傷修復(fù)實(shí)驗(yàn)、炎癥反應(yīng)評(píng)估或血管生成相關(guān)機(jī)制研究,為探索PDGF - AB在組織再生、病理進(jìn)程中的調(diào)控作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):PDGF-AB
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:7.8 pg/mL-500 pg/mL
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靈敏度:1.95 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cancer
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 91-99 1:2 Average % 88 Range % 84-93 1:4 Average % 105 Range % 101-109 1:8 Average % 100 Range % 95-106 -
回收率:
The recovery of rat PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 87 83-90 EDTA plasma (n=4) 102 97-106 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 500 1.978 2.012 1.995 1.827 250 1.568 1.601 1.585 1.417 125 1.016 1.032 1.024 0.856 62.5 0.527 0.518 0.523 0.355 31.2 0.354 0.361 0.358 0.190 15.6 0.257 0.248 0.253 0.085 7.8 0.204 0.211 0.208 0.040 0 0.166 0.169 0.168 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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