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  4. Rat Homocysteic acid,Hcy ELISA Kit

Rat Homocysteic acid,Hcy ELISA Kit

  • 中文名稱：
    大鼠同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08896r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E08896r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝過(guò)程中的中間產(chǎn)物。其水平升高被視為心腦血管疾病等多種病癥的獨(dú)立危險(xiǎn)因素。研究機(jī)制主要集中在其對(duì)血管內(nèi)皮細(xì)胞的損傷、促進(jìn)血栓形成及氧化應(yīng)激反應(yīng)等方面，深入研究它有助于疾病防治。試劑盒檢測(cè)范圍為0.78 nmol/mL-50 nmol/mL，適用于科研場(chǎng)景下對(duì)大鼠模型體內(nèi)Hcy代謝水平的動(dòng)態(tài)監(jiān)測(cè)，例如營(yíng)養(yǎng)代謝研究、藥物毒性評(píng)價(jià)或疾病機(jī)制探索?？蓮V泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及動(dòng)物模型構(gòu)建等領(lǐng)域，助力Hcy相關(guān)分子機(jī)制的深入解析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    N/A
  • 縮寫：
    Hcy
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.78 nmol/mL-50 nmol/mL
  • 靈敏度：
    0.195 nmol/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %104
    Range %100-108
    1:2Average %96
    Range %92-100
    1:4Average %94
    Range %90-98
    1:8Average %89
    Range %84-93
  • 回收率：
    The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9794-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/ml. OD1OD2AverageCorrected
    502.331 2.368 2.350 2.218
    251.626 1.637 1.632 1.500
    12.51.089 1.074 1.082 0.950
    6.250.679 0.678 0.679 0.547
    3.120.467 0.445 0.456 0.324
    1.560.341 0.368 0.355 0.223
    0.780.285 0.268 0.277 0.145
    00.131 0.133 0.132 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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