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  4. Rat Homocysteine(Hcy)ELISA Kit

Rat Homocysteine(Hcy)ELISA Kit

  • 中文名稱：
    大鼠高半胱氨酸(Hcy)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13376r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠高半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E13376r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝的中間產(chǎn)物。其水平升高是心腦血管疾病等多種疾病的獨(dú)立危險因素。研究機(jī)制上，可能通過損傷血管內(nèi)皮細(xì)胞、促進(jìn)血栓形成、氧化應(yīng)激等途徑參與疾病發(fā)生發(fā)展，對其深入研究有重要臨床意義。試劑盒檢測范圍為0.78 nmol/mL-50 nmol/mL，廣泛應(yīng)用于大鼠模型的基礎(chǔ)研究，例如在高血壓、動脈粥樣硬化或阿爾茨海默病等動物模型中評估Hcy水平變化，或用于探究營養(yǎng)干預(yù)、藥物代謝對Hcy調(diào)控的影響，為疾病機(jī)制解析及治療策略開發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    Hcy
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 nmol/mL-50 nmol/mL
  • 靈敏度：
    0.195 nmol/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-95
    1:2Average %94
    Range %90-98
    1:4Average %94
    Range %89-97
    1:8Average %100
    Range %93-103
  • 回收率：
    The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9592-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/mlOD1OD2AverageCorrected
    502.874 2.771 2.823 2.670
    252.223 2.245 2.234 2.081
    12.51.418 1.389 1.404 1.251
    6.250.756 0.775 0.766 0.613
    3.120.462 0.448 0.455 0.302
    1.560.321 0.313 0.317 0.164
    0.780.232 0.225 0.229 0.076
    00.155 0.151 0.153 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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