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  4. Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

Rat triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

  • 中文名稱：
    大鼠髓樣細(xì)胞觸發(fā)性受體2(TREM2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL024405RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠髓樣細(xì)胞觸發(fā)性受體2(TREM2)酶聯(lián)免疫試劑盒（CSB-EL024405RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TREM2含量。TREM2是一種跨膜受體，主要表達(dá)于小膠質(zhì)細(xì)胞，其作用機(jī)制涉及感知組織損傷、介導(dǎo)免疫重塑和調(diào)控小膠質(zhì)細(xì)胞功能。研究發(fā)現(xiàn)，TREM2與多種配體結(jié)合后，可激活下游信號(hào)通路，影響小膠質(zhì)細(xì)胞增殖、吞噬和炎癥反應(yīng)，從而參與多種疾病如阿爾茨海默病、代謝綜合征和腫瘤的病理進(jìn)程。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于神經(jīng)免疫機(jī)制研究、小膠質(zhì)細(xì)胞功能探索、阿爾茨海默病或代謝相關(guān)疾病的動(dòng)物模型分析等科研場景，為揭示TREM2在病理生理過程中的作用提供可靠工具，實(shí)驗(yàn)結(jié)果需結(jié)合其他研究手段進(jìn)行綜合分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on monocytes 2|triggering receptor expressed on myeloid cells 2a
  • 縮寫：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %106
    Range %101-115
    1:4Average %88
    Range %84-92
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of rat TREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9288-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.660 2.754 2.707 2.530
    5001.957 2.006 1.982 1.805
    2501.309 1.293 1.301 1.124
    1250.919 0.937 0.928 0.751
    62.50.482 0.501 0.492 0.315
    31.20.407 0.425 0.416 0.239
    15.60.247 0.238 0.243 0.066
    00.174 0.180 0.177
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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