Sheep adrenocorticotropic hormone (ACTH) ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

綿羊促腎上腺皮質激素(ACTH)酶聯(lián)免疫試劑盒（CSB-EQ027618SH）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的ACTH含量。促腎上腺皮質激素（ACTH）是腺垂體分泌的多肽激素。其它對維持腎上腺正常形態(tài)和功能有重要作用。研究機制上，ACTH作用于腎上腺皮質束狀帶，刺激糖皮質激素合成與分泌，在應激反應等生理過程中發(fā)揮關鍵調節(jié)作用。試劑盒檢測范圍為0.097 pg/mL-400 pg/mL，適用于綿羊等反芻動物應激狀態(tài)評估、腎上腺功能研究、藥理實驗中激素水平動態(tài)監(jiān)測等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

ACTH
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測范圍：

0.097 pg/mL-400 pg/mL
靈敏度：

0.097 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	91
	Range %	88-95
1:4	Average %	87
	Range %	83-95
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	89	87-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.