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malE Recombinant Monoclonal Antibody

  • 中文名稱：
    malE重組抗體
  • 貨號(hào)：
    CSB-RA846828A0ENV
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Escherichia coli lysate
      All lanes: malE antibody at 1: 1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 44 kDa
      Observed band size: 44 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA846828A0ENV malE重組單克隆抗體是一款經(jīng)ELISA及Western Blot（WB）驗(yàn)證的高特異性科研工具，靶向細(xì)菌麥芽糖結(jié)合蛋白（MalE/MBP）。該抗體通過(guò)重組表達(dá)技術(shù)制備，可精準(zhǔn)識(shí)別原核表達(dá)系統(tǒng)中表達(dá)的MalE蛋白，適用于檢測(cè)帶有MBP標(biāo)簽的融合蛋白及分析細(xì)菌麥芽糖轉(zhuǎn)運(yùn)相關(guān)機(jī)制。在驗(yàn)證實(shí)驗(yàn)中，抗體在WB中呈現(xiàn)單一清晰的目標(biāo)條帶，且ELISA檢測(cè)顯示線性范圍寬泛、靈敏度優(yōu)異，適用于低豐度樣本分析。其應(yīng)用場(chǎng)景涵蓋分子生物學(xué)研究中重組蛋白純化工藝的質(zhì)控、原核表達(dá)系統(tǒng)蛋白表達(dá)水平的檢測(cè)，以及微生物代謝途徑相關(guān)蛋白的功能研究。產(chǎn)品經(jīng)嚴(yán)格質(zhì)檢，具有批次穩(wěn)定性和高重復(fù)性，可為細(xì)菌蛋白互作、原核表達(dá)體系優(yōu)化等基礎(chǔ)研究提供可靠支持。
  • Uniprot No.：
  • 基因名：
    malE
  • 別名：
    Maltose-binding periplasmic protein (MBP) (MMBP) (Maltodextrin-binding protein), malE
  • 反應(yīng)種屬：
    Escherichia coli
  • 免疫原：
    A synthesized peptide derived from Escherichia coli malE
  • 免疫原種屬：
    Escherichia coli (strain K12)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    10F2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
  • 基因功能參考文獻(xiàn)：
    1. Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-like folding intermediate that brings together sequentially remote segments and then folds globally after a long delay. PMID: 29295923
    2. Our current understanding of what makes MBP such an effective solubility enhancer for other proteins and how it facilitates the proper folding of its fusion partners is presented [REVIEW] PMID: 28132493
    3. Maltose Binding Protein is key to the coupling of maltose transport to ATP hydrolysis in vivo, because it facilitates the progression of the MalK dimer from the open to the semi-open conformation, from which it can proceed to hydrolyze ATP PMID: 26268698
    4. activates mouse macrophages and polarizes them into M1 macrophages PMID: 24825603
    5. a salt-bridge motif in the maltose-binding protein has a role in the initial steps of substrate transport PMID: 16143635
    6. Our findings indicate that the solubility-enhancing activity of MBP is mediated by its open conformation and point to a likely role for the ligand-binding cleft in the mechanism of solubility enhancement. PMID: 17964542
    7. These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB. PMID: 18241889
    8. tat mutants are able to transport maltose in the absence of malE. PMID: 18385983

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  • 亞細(xì)胞定位：
    Periplasm.
  • 蛋白家族：
    Bacterial solute-binding protein 1 family
  • 數(shù)據(jù)庫(kù)鏈接：