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SAE1 Recombinant Monoclonal Antibody

  • 中文名稱：
    SAE1重組抗體
  • 貨號(hào)：
    CSB-RA987582A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate
      All lanes: SAE1 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 39, 30, 34 kDa
      Observed band size: 39 kDa
    • Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA987582A0HU SAE1重組單克隆抗體是針對(duì)SUMO激活酶復(fù)合物關(guān)鍵亞基SAE1研發(fā)的高特異性檢測(cè)工具。該抗體靶向的SAE1蛋白參與調(diào)控SUMO化修飾過程，這一翻譯后修飾機(jī)制在細(xì)胞周期進(jìn)程、DNA損傷修復(fù)及蛋白定位等基礎(chǔ)生物學(xué)過程中發(fā)揮重要作用。經(jīng)多種技術(shù)平臺(tái)驗(yàn)證，本產(chǎn)品在Western Blot（WB）和免疫熒光（IF）實(shí)驗(yàn)中展現(xiàn)出優(yōu)異性能，推薦使用濃度分別為1:500-1:5000和1:20-1:200，其高親和力可清晰識(shí)別內(nèi)源性SAE1蛋白表達(dá)。在ELISA體系中同樣具備穩(wěn)定檢測(cè)能力，適用于多種實(shí)驗(yàn)體系的需求?？蒲腥藛T可利用該抗體深入探索SUMO化修飾通路在腫瘤發(fā)生、神經(jīng)退行性疾病等病理生理模型中的作用機(jī)制，為蛋白質(zhì)翻譯后修飾研究、細(xì)胞應(yīng)激反應(yīng)分析及藥物靶點(diǎn)篩選提供可靠工具。產(chǎn)品嚴(yán)格通過質(zhì)控驗(yàn)證，確保批次間穩(wěn)定性，滿足細(xì)胞生物學(xué)、分子機(jī)制研究等領(lǐng)域的實(shí)驗(yàn)需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human SAE1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    9D8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • 基因功能參考文獻(xiàn)：
    1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
    2. as it has been shown by flow cytometry analysis, specific knockdown of SAE1 slowed down the cell population at G0/G1 phase and induced apoptosis of RKO cells. PMID: 25842831
    3. SUMO-1 controls the protein stability and the biological function of phosducin. PMID: 16421094
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Ubiquitin-activating E1 family
  • 組織特異性：
    Expression level increases during S phase and drops in G2 phase (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 30660

    OMIM: 613294

    KEGG: hsa:10055

    STRING: 9606.ENSP00000270225

    UniGene: Hs.515500