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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella melitensis biotype 2 Integration host factor subunit beta (ihfB) CSB-YP499689BWT
CSB-EP499689BWT
CSB-BP499689BWT
CSB-MP499689BWT
CSB-EP499689BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Thiazole synthase (thiG) CSB-YP499690BWT
CSB-EP499690BWT
CSB-BP499690BWT
CSB-MP499690BWT
CSB-EP499690BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Dihydroorotate dehydrogenase (quinone) CSB-YP499691BWT
CSB-EP499691BWT
CSB-BP499691BWT
CSB-MP499691BWT
CSB-EP499691BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP499692BWT
CSB-EP499692BWT
CSB-BP499692BWT
CSB-MP499692BWT
CSB-EP499692BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 HTH-type transcriptional regulator BetI (betI) CSB-YP499693BWT
CSB-EP499693BWT
CSB-BP499693BWT
CSB-MP499693BWT
CSB-EP499693BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP499694BWT
CSB-EP499694BWT
CSB-BP499694BWT
CSB-MP499694BWT
CSB-EP499694BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Putative arginyl-tRNA--protein transferase (ate) CSB-YP499695BWT
CSB-EP499695BWT
CSB-BP499695BWT
CSB-MP499695BWT
CSB-EP499695BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP499696BWT
CSB-EP499696BWT
CSB-BP499696BWT
CSB-MP499696BWT
CSB-EP499696BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP499697BWT
CSB-EP499697BWT
CSB-BP499697BWT
CSB-MP499697BWT
CSB-EP499697BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 5'-nucleotidase surE (surE) CSB-YP499698BWT
CSB-EP499698BWT
CSB-BP499698BWT
CSB-MP499698BWT
CSB-EP499698BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP499699BWT
CSB-EP499699BWT
CSB-BP499699BWT
CSB-MP499699BWT
CSB-EP499699BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP499700BWT
CSB-EP499700BWT
CSB-BP499700BWT
CSB-MP499700BWT
CSB-EP499700BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 LexA repressor (lexA) CSB-YP499701BWT
CSB-EP499701BWT
CSB-BP499701BWT
CSB-MP499701BWT
CSB-EP499701BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP499702BWT
CSB-EP499702BWT
CSB-BP499702BWT
CSB-MP499702BWT
CSB-EP499702BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L15 (rplO) CSB-YP499703BWT
CSB-EP499703BWT
CSB-BP499703BWT
CSB-MP499703BWT
CSB-EP499703BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L24 (rplX) CSB-YP499704BWT
CSB-EP499704BWT
CSB-BP499704BWT
CSB-MP499704BWT
CSB-EP499704BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L23 (rplW) CSB-YP499705BWT
CSB-EP499705BWT
CSB-BP499705BWT
CSB-MP499705BWT
CSB-EP499705BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP499706BWT
CSB-EP499706BWT
CSB-BP499706BWT
CSB-MP499706BWT
CSB-EP499706BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP499707BWT
CSB-EP499707BWT
CSB-BP499707BWT
CSB-MP499707BWT
CSB-EP499707BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 60 kDa chaperonin (groL), partial CSB-YP499708BWT
CSB-EP499708BWT
CSB-BP499708BWT
CSB-MP499708BWT
CSB-EP499708BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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