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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Wolbachia sp. subsp. Drosophila simulans Leucine--tRNA ligase (leuS), partial CSB-YP499669WBP
CSB-EP499669WBP
CSB-BP499669WBP
CSB-MP499669WBP
CSB-EP499669WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP499670WBP
CSB-EP499670WBP
CSB-BP499670WBP
CSB-MP499670WBP
CSB-EP499670WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Protein-export protein secB (secB) CSB-YP499671WBP
CSB-EP499671WBP
CSB-BP499671WBP
CSB-MP499671WBP
CSB-EP499671WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Enolase (eno) CSB-YP499672WBP
CSB-EP499672WBP
CSB-BP499672WBP
CSB-MP499672WBP
CSB-EP499672WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans ATP synthase subunit c (atpE), partial CSB-YP499673WBP1
CSB-EP499673WBP1
CSB-BP499673WBP1
CSB-MP499673WBP1
CSB-EP499673WBP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0303 protein BMEA_A1459 (BMEA_A1459) CSB-YP499674BWT
CSB-EP499674BWT
CSB-BP499674BWT
CSB-MP499674BWT
CSB-EP499674BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP499675BWT
CSB-EP499675BWT
CSB-BP499675BWT
CSB-MP499675BWT
CSB-EP499675BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Transcription elongation factor GreA (greA) CSB-YP499676BWT
CSB-EP499676BWT
CSB-BP499676BWT
CSB-MP499676BWT
CSB-EP499676BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Malate synthase G (glcB), partial CSB-YP499677BWT
CSB-EP499677BWT
CSB-BP499677BWT
CSB-MP499677BWT
CSB-EP499677BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP499678BWT
CSB-EP499678BWT
CSB-BP499678BWT
CSB-MP499678BWT
CSB-EP499678BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP499679BWT
CSB-EP499679BWT
CSB-BP499679BWT
CSB-MP499679BWT
CSB-EP499679BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Protease HtpX homolog (htpX), partial CSB-YP499680BWT1
CSB-EP499680BWT1
CSB-BP499680BWT1
CSB-MP499680BWT1
CSB-EP499680BWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Gamma-glutamyl phosphate reductase (proA) CSB-YP499681BWT
CSB-EP499681BWT
CSB-BP499681BWT
CSB-MP499681BWT
CSB-EP499681BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP499682BWT
CSB-EP499682BWT
CSB-BP499682BWT
CSB-MP499682BWT
CSB-EP499682BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0178 protein BMEA_A2036 (BMEA_A2036) CSB-YP499683BWT
CSB-EP499683BWT
CSB-BP499683BWT
CSB-MP499683BWT
CSB-EP499683BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP499684BWT
CSB-EP499684BWT
CSB-BP499684BWT
CSB-MP499684BWT
CSB-EP499684BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Tryptophan synthase alpha chain (trpA) CSB-YP499685BWT
CSB-EP499685BWT
CSB-BP499685BWT
CSB-MP499685BWT
CSB-EP499685BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Chaperone protein DnaJ (dnaJ) CSB-YP499686BWT
CSB-EP499686BWT
CSB-BP499686BWT
CSB-MP499686BWT
CSB-EP499686BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP499687BWT
CSB-EP499687BWT
CSB-BP499687BWT
CSB-MP499687BWT
CSB-EP499687BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP499688BWT
CSB-EP499688BWT
CSB-BP499688BWT
CSB-MP499688BWT
CSB-EP499688BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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